mouse il 21 gene  (InvivoGen)

Journal: Nature Communications

Article Title: Hepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance

doi: 10.1038/s41467-017-02304-7

Figure Lengend Snippet: Serum IL-21 and IL-33 are stimulated in B6 HDI mice but not BPS HDI mice. Sera from BPS, B6 and pUC18 HDI BALB/c mice were collected at indicated time points and levels of selected cytokines quantitated using multiplex-based assay. Group means and s.e.m. within group of serum IL-21 ( a ) and IL-33 ( b ) measurements are presented with group sizes ( n ) indicated. BPS and B6 mice data are compared against pUC18 mice and statistical significance calculated using unpaired two-tailed t -test. * p < 0.05; ** p < 0.01. For data on other tested cytokines, see Supplementary Fig. . w.p.i. weeks post injection

Article Snippet: Mouse IL-21 (MG50137-M-N) and IL-33 (MG50118-M-N) expression plasmids (Sino Biological, China) and rat monoclonal antibodies (anti-mIL-21: 16-9333; anti-mIL-33, 16-7211) (eBioscience, China) were purchased.

Techniques: Multiplex Assay, Two Tailed Test, Injection

Journal: Nature Communications

Article Title: Hepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance

doi: 10.1038/s41467-017-02304-7

Figure Lengend Snippet: IL-21 or IL-33 treatments induce clearance of established BPS persistence. Plasmids expressing murine IL-21 or IL-33 and empty vector control were injected into BPS HDI mice at 4 and 7 weeks post injection (w.p.i.) (arrows). Sera were collected at indicated time points and levels of IL-21 and IL-33 ( a ), HBsAg ( b ), HBsAb ( c ) and HBeAg ( d ) were analysed using ELISA. Group means and s.e.m. within group are presented with group sizes ( n ) indicated. Group positivity percentage data are also presented for HBsAg ( b , right panel), HBsAb ( c , right panel) and HBeAg ( d , right panel). HBV DNA for each group were analysed using pooled serum in commercial quantitative assay ( e ). Dotted lines represent cut-off thresholds ( a , b – d , right panels) and lower limit of quantification ( e ) respectively. geq genome equivalents

Article Snippet: Mouse IL-21 (MG50137-M-N) and IL-33 (MG50118-M-N) expression plasmids (Sino Biological, China) and rat monoclonal antibodies (anti-mIL-21: 16-9333; anti-mIL-33, 16-7211) (eBioscience, China) were purchased.

Techniques: Expressing, Plasmid Preparation, Injection, Enzyme-linked Immunosorbent Assay

Journal: Nature Communications

Article Title: Hepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance

doi: 10.1038/s41467-017-02304-7

Figure Lengend Snippet: IL-21-induced or IL-33-induced clearance of BPS persistence engenders protection from re-challenge. HDI mice harbouring BPS persistence were treated with injections of plasmids expressing IL-21 ( a ) or IL-33 ( b ) at 4 and 7 w.p.i. and those displaying at least 4 weeks of sustained HBsAg negativity (see Fig. ) were re-challenged in the same way as initial BPS HDI. BPS HDI mice treated with vector control were used as control and re-challenged similarly ( c ). Sera were collected at indicated time points and levels of HBsAg (left), HBsAb (middle) and HBeAg (right) were analysed using ELISA. Dotted lines represent cut-off thresholds. w.p.i. weeks post injection of BPS re-challenge

Article Snippet: Mouse IL-21 (MG50137-M-N) and IL-33 (MG50118-M-N) expression plasmids (Sino Biological, China) and rat monoclonal antibodies (anti-mIL-21: 16-9333; anti-mIL-33, 16-7211) (eBioscience, China) were purchased.

Techniques: Expressing, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Injection